Continuous bromodeoxyuridine labeling and bivariate ethidium bromide/hoechst flow cytometry in cell kinetics
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چکیده
منابع مشابه
Cell kinetics in human malignancies studied with in vivo administration of bromodeoxyuridine and flow cytometry.
Bromodeoxyuridine (BrdUrd) is a pyrimidine analogue which is incorporated into the DNA of proliferating cells. When in vivo BrdUrd infusion is coupled with bivariate flow cytometry to measure cell BrdUrd incorporation and DNA content, both the percentage of DNA-synthesizing cells [BrdUrd-labeling index (LI)] and the DNA synthesis time (TS) can be determined on the same tissue sample. From exper...
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متن کاملCell Kinetics in Human Malignancies Studied with in Vivo Administration of Bromodeoxyuridine and Flow Cytometry1
Bromodeoxyuridine (BrdUrd) is a pyrimidine analogue which is incor porated into the DN A of proliferating cells. When in vivoBrdUrd infusion is coupled with Divariate flow cytomctry to measure cell BrdUrd incor poration and I)V\ content, both the percentage of DNA-synthesizing cells jBrdUrd-labeling index (LI)] and the DNA synthesis time (Ts) can be determined on the same tissue sample. From ex...
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Non-radioisotopic local lymph node assay (LLNA) employing 5-bromo-2'-deoxyuridine (BrdU) with flow cytometry (FACS) or immunohistochemistry (IHC) is gaining attention due to a regulatory issue of using radioisotope, (3)H-thymidine, in vivo in traditional LLNA. In this study, to compare the performance of these non-radioisotopic endpoints, 7 chemicals with known sensitizing potencies were examin...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1989
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990100215